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[NOVEL APPROACH TO COMPOSITION OF, BACTERIOPHAGE MIXTURES FOR ANTIBACTERIAL THERAPY]

E A Pleteneva, O V Shaburova, M V Burkaltseva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 30, 2019
    PubMed
    Abstract

    Insights

    This study demonstrates that experimental mixtures of six bacteriophage species exhibit potent antibacterial activity against Pseudomonas aeruginosa. These phage mixtures show comparable efficacy to commercial preparations, offering a promising alternative for treating infections.

    Area of Science:

    • Microbiology
    • Bacteriophage Therapy
    • Antimicrobial Resistance

    Background:

    • Pseudomonas aeruginosa is a significant opportunistic pathogen causing various infections.
    • The rise of antibiotic resistance necessitates alternative therapeutic strategies.
    • Bacteriophages offer a potential solution for combating bacterial infections.

    Purpose of the Study:

    • To evaluate the antibacterial activity of an experimental mixture of bacteriophages against clinical isolates of Pseudomonas aeruginosa.
    • To compare the efficacy of the experimental phage mixture with commercial therapeutic preparations.

    Main Methods:

    • Utilized 55 clinical Pseudomonas aeruginosa strains.
    • Prepared mono-species mixtures of 32 virulent bacteriophages from several well-studied species, plus two novel phages.
    • Assessed lytic activity using standard methods, including seeding onto bacterial lawns and restriction analysis of phage DNA.

    Main Results:

    • The cumulative activity of six mono-species phage mixtures was comparable to commercial therapeutic mixtures.
    • 54 out of 55 clinical P. aeruginosa strains showed sensitivity to the experimental phage mixtures.
    • 53 strains were lysed by commercial preparations, with no accidental inclusion of moderate phages in the experimental mixture.

    Conclusions:

    • Highly active therapeutic antibacterial preparations against P. aeruginosa can be created using mono-species mixtures of lytic bacteriophages.
    • Utilizing such phage mixtures in lung infection therapy may reduce the risk of bacterial strain virulence and pathogenicity during prolonged treatment.

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