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Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Lytic Cycle of Bacteriophages01:30

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Mixtures of Acids03:27

Mixtures of Acids

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The pH of a solution containing an acid can be determined using its acid dissociation constant and its initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending upon the relative strength of the acids and their dissociation constants.
A Mixture of a Strong Acid and a Weak Acid
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Mixtures of Acids01:19

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The pH of a solution containing an acid can be determined using its acid dissociation constant and initial concentration. If a solution contains two different acids, then its pH can be determined using one of several methods depending on the relative strength of the acids and their dissociation constants.
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Classifying Matter by Composition03:35

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Matter: Pure Substances and Mixtures
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DNA Bacteriophages01:26

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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[NOVEL APPROACH TO COMPOSITION OF, BACTERIOPHAGE MIXTURES FOR ANTIBACTERIAL THERAPY].

E A Pleteneva, O V Shaburova, M V Burkaltseva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 30, 2019
    PubMed
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    This study demonstrates that experimental mixtures of six bacteriophage species exhibit potent antibacterial activity against Pseudomonas aeruginosa. These phage mixtures show comparable efficacy to commercial preparations, offering a promising alternative for treating infections.

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    Area of Science:

    • Microbiology
    • Bacteriophage Therapy
    • Antimicrobial Resistance

    Background:

    • Pseudomonas aeruginosa is a significant opportunistic pathogen causing various infections.
    • The rise of antibiotic resistance necessitates alternative therapeutic strategies.
    • Bacteriophages offer a potential solution for combating bacterial infections.

    Purpose of the Study:

    • To evaluate the antibacterial activity of an experimental mixture of bacteriophages against clinical isolates of Pseudomonas aeruginosa.
    • To compare the efficacy of the experimental phage mixture with commercial therapeutic preparations.

    Main Methods:

    • Utilized 55 clinical Pseudomonas aeruginosa strains.
    • Prepared mono-species mixtures of 32 virulent bacteriophages from several well-studied species, plus two novel phages.
    • Assessed lytic activity using standard methods, including seeding onto bacterial lawns and restriction analysis of phage DNA.

    Main Results:

    • The cumulative activity of six mono-species phage mixtures was comparable to commercial therapeutic mixtures.
    • 54 out of 55 clinical P. aeruginosa strains showed sensitivity to the experimental phage mixtures.
    • 53 strains were lysed by commercial preparations, with no accidental inclusion of moderate phages in the experimental mixture.

    Conclusions:

    • Highly active therapeutic antibacterial preparations against P. aeruginosa can be created using mono-species mixtures of lytic bacteriophages.
    • Utilizing such phage mixtures in lung infection therapy may reduce the risk of bacterial strain virulence and pathogenicity during prolonged treatment.