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Updated: Jan 30, 2026

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

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[HBV COVALENTLY CLOSED CIRCULAR DNA AS A MARKER OF PREVALENCE OF OCCULT HEPATITIS B IN PATIENTS WITH HBV, HDVAND HCV

A V Semenov, Yu V Ostankova, Kh N Faizullaev

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 30, 2019
    PubMed

    Insights

    Hepatitis B virus covalently closed circular DNA (cccDNA) is a significant marker for detecting occult hepatitis B in Uzbekistan patients with various liver diseases. Its presence aids in diagnosis, treatment, and prognosis.

    Area of Science:

    • Hepatology
    • Virology
    • Molecular Diagnostics

    Background:

    • Occult hepatitis B virus (HBV) infection poses diagnostic challenges.
    • Covalently closed circular DNA (cccDNA) is a key form of HBV DNA in infected cells.
    • Understanding HBV cccDNA's role is crucial for managing liver diseases.

    Purpose of the Study:

    • To evaluate the significance of HBV cccDNA as a marker for detecting occult HBV.
    • To assess HBV cccDNA in Uzbekistani patients with hepatitis of various etiologies.

    Main Methods:

    • Study material included blood plasma and liver biopsy from 39 patients.
    • HBV cccDNA detection was performed using established methods (Pollicino T et al., 2004).

    Main Results:

    • HBV cccDNA was detected in 82% of all samples analyzed.
    • cccDNA was found in 54.5% of chronic viral hepatitis C patients and 100% of those with unknown etiology.
    • Quantitative analysis revealed varying cccDNA levels across different hepatitis types.

    Conclusions:

    • HBV cccDNA detection is vital for diagnosing occult hepatitis B.
    • It is an important factor for guiding therapy, prognosis, and preventing severe liver disease progression.
    Abstract

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