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The interaction between P2X7Rs and T-type calcium ion channels in penicillin-induced epileptiform activity

Gokhan Arslan1, Bahattin Avci2, Süleyman Emre Kocacan1

  • 1Department of Physiology, Medical School, University of Ondokuz Mayis, Samsun, Turkey.

Neuropharmacology
|January 30, 2019
PubMed

Insights

Purinergic P2X7 receptors (P2X7Rs) influence epilepsy by interacting with T-type calcium channels. Blocking these channels reduces seizure activity and oxidative stress, suggesting a therapeutic target for epilepsy.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • Limited understanding of the interplay between P2X7 receptors and calcium channels in epilepsy.
  • No prior research on the specific interaction between P2X7 receptors and T-type calcium channels in epilepsy models.

Purpose of the Study:

  • To investigate the role of T-type calcium channels in the effects of P2X7 receptors on penicillin-induced epileptiform activity.
  • To evaluate the impact of P2X7 receptor modulation and T-type calcium channel blockade on oxidative stress markers in an epilepsy model.

Main Methods:

  • Electrophysiological recordings were performed in rats following penicillin injection.
  • Administration of P2X7 receptor agonist (BzATP) and antagonists (A-438079), and T-type calcium channel antagonist (NNC-550396).
  • Biochemical analysis of oxidative stress markers (MDA, AOPP, GSH, GR, GPx, CAT, SOD) in rat brain regions.

Main Results:

  • P2X7 receptor activation increased epileptiform activity, while its antagonist and T-type calcium channel antagonist reduced it.
  • Both antagonists counteracted the proconvulsant effects of BzATP.
  • BzATP induced lipid and protein oxidation, which were reversed by the antagonists, particularly in the cerebrum.

Conclusions:

  • P2X7 receptors and their interaction with T-type calcium channels are crucial in an experimental epilepsy model.
  • Targeting P2X7 receptors and T-type calcium channels may offer a novel therapeutic strategy for epilepsy management.
Keywords:
Antioxidant enzymesExperimental epilepsyLipid peroxidationP2X7 receptorsProtein oxidationT-type calcium ion channels

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