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Updated: Jan 30, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
Published on: August 31, 2012
Propargylated Purine Deoxynucleosides: New Tools for Fluorescence Imaging Strategies
Akkaladevi Venkatesham1, Sambasiva Rao Pillalamarri2, Flore De Wit3
1Medicinal Chemistry, Rega Institute for Medical Research, Dept. of Pharmaceutical and Pharmacological Sciences, KU Leuven, Herestraat 49, 3000 Leuven, Belgium. venkat.pallavi2013@gmail.com.
Abstract:
In vivo imaging of biological processes is an important asset of modern cell biology. Selectively reacting fluorophores herein are an important tool and click chemistry reactions take a large share in these events. 5-Ethynyl-2'-deoxyuridine (EdU) is well known for visualizing DNA replication, but does not show any selectivity for incorporation into DNA. Striving for specific visualization of virus replication, in particular HIV replication, a series of propargylated purine deoxynucleosides were prepared aiming for selective incorporation by HIV reverse transcriptase (RT). We here report on the synthesis and preliminary biological effects (cellular toxicity, HIV inhibitory effects, and feasibility of the click reaction) of these nucleoside analogues.
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