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MreB Forms Subdiffraction Nanofilaments during Active Growth in Bacillus subtilis.

Cyrille Billaudeau1, Zhizhong Yao1, Charlène Cornilleau2

  • 1MICALIS, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.

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MreB protein forms short nanofilaments during bacterial growth, not long ones as previously thought. Filament length does not affect bacterial cell wall synthesis dynamics.

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Area of Science:

  • Bacterial cell shape and growth
  • Microbial cell envelope biogenesis

Background:

  • MreB protein is crucial for rod-shaped bacterial cell elongation and maintenance.
  • MreB dynamics and filament structures are debated, impacting models of cell wall synthesis.

Purpose of the Study:

  • To clarify the native structure and dynamics of MreB filaments in *Bacillus subtilis*.
  • To investigate the relationship between MreB filament length and bacterial growth dynamics.

Main Methods:

  • Analysis of MreB filament formation in various *Bacillus subtilis* strains.
  • Microscopy techniques to observe filament size, speed, and orientation during active growth.

Main Results:

  • MreB forms short, subdiffraction-limited nanofilaments (average <200 nm) during active growth.
  • Longer, micron-long filaments result from artificial protein overaccumulation.
  • Filament size does not impact their speed or orientation, showing high tolerance in *B. subtilis*.
  • Mobile filament density is constant across strains, irrespective of MreB levels.

Conclusions:

  • Native MreB filaments in *B. subtilis* are short, challenging models of long filament coordination.
  • Filament length does not directly influence cell wall synthesis rates or coordination of distant machineries.