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A Magnaporthe Chitinase Interacts with a Rice Jacalin-Related Lectin to Promote Host Colonization

Yijuan Han1,2,3, Linlin Song1,3, Changlin Peng1,3

  • 1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Plant Physiology
|January 31, 2019
PubMed

Insights

The rice blast fungus chitinase MoChi1 suppresses rice immunity by interacting with the host lectin OsMBL1. This interaction inhibits defense responses, highlighting a novel fungal virulence strategy against rice.

Area of Science:

  • Plant-pathogen interactions
  • Molecular plant pathology
  • Fungal virulence mechanisms

Background:

  • Rice blast fungus (Magnaporthe oryzae) is a major pathogen of rice.
  • Glycoside hydrolase family 18 chitinases are secreted by M. oryzae.
  • Host lectins like OsMBL1 play roles in plant immunity.

Purpose of the Study:

  • To characterize the function of M. oryzae extracellular chitinase MoChi1.
  • To investigate the interaction between MoChi1 and rice OsMBL1.
  • To elucidate the role of MoChi1-OsMBL1 interaction in rice-M. oryzae interactions.

Main Methods:

  • Gene deletion and overexpression in M. oryzae and rice.
  • In vitro and in vivo interaction assays (e.g., co-immunoprecipitation).
  • Analysis of defense gene expression, reactive oxygen species (ROS) burst, and plant susceptibility.

Main Results:

  • Deletion of MoChi1 reduced fungal virulence and aerial hyphal formation.
  • MoChi1 directly interacts with rice OsMBL1.
  • OsMBL1 overexpression enhanced rice resistance, while knockdown increased susceptibility.
  • MoChi1 suppressed chitin-induced ROS burst and competed with OsMBL1 for chitin binding.

Conclusions:

  • MoChi1 targets the host lectin OsMBL1 to suppress rice immunity.
  • This interaction is a key mechanism for M. oryzae virulence.
  • Understanding this interaction can lead to novel strategies for controlling rice blast disease.

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