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Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles
Published on: June 2, 2017
Comparative evaluation of antibacterial effect of nanoparticles and lasers against Endodontic Microbiota: An in vitro
Vibha Kushwaha1, Rakesh-Kumar Yadav2, Aseem-Prakash Tikku3
1Junior Resident, Department of Conservative Dentistry and Endodontics, King George's Medical University, Lucknow, U.P., India.
Background:
Present study was conducted with the aim of evaluating antimicrobial efficacy of silver (AgNP) and gold nanoparticles (AuNP) with and without Nd: YAG laser (L) irradiation against experimentally inoculated Enterococcus faecalis in infected human root dentin.
Material And Methods:
120 extracted single rooted human teeth were prepared and inoculated with E. faecalis for 24 hrs. The teeth were then randomly divided into 4 experimental group: AgNPs group: irrigation for 3minutes with 50 μl of 100 ppm, the AuNPs group: irrigation with 50 μl of 100 ppm, the AgNPs & Nd: YAG lasers group: irrigation with 50 μl of 100 ppm + irradiation with 1.5W laser for 60 seconds, the AuNPs & Nd: YAG lasers group: irrigation with 50 μl of 100 ppm + irradiation with 1.5W laser for 60 seconds. One control group consisting of 2% CHX irrigation for 3 minutes was also there (n = 20). The specimens were collected from the canal before and after irrigation, and colony forming units were observed.
Results:
Significant difference was found among all the groups in comparison to the control group (p<0.05). The greatest reduction in CFU's was observed with combination of AgNPs & Nd: YAG lasers group.
Conclusions:
AgNPs in combination with Nd: YAG laser irradiation has the potential to be used as root canal disinfectant. Key words:Antibacterial efficacy, gold, silver, nanoparticles, lasers, solid-state.
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