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Updated: Jan 30, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

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PentraSorb C-Reactive Protein: Characterization of the Selective C-Reactive Protein Adsorber Resin

Stephan Mattecka1, Patrizia Brunner2, Britta Hähnel1

  • 1Pentracor GmbH, Hennigsdorf, Germany.

Therapeutic Apheresis and Dialysis : Official Peer-Reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy
|January 31, 2019
PubMed

Insights

A novel PentraSorb CRP adsorber effectively removes C-reactive protein (CRP), a key inflammation marker and cardiac risk factor, from human plasma. This technology shows promise for treating acute myocardial infarction and other inflammatory conditions.

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Immunology

Background:

  • C-reactive protein (CRP) is a significant marker of inflammation and a risk factor for cardiac events, including acute myocardial infarction (AMI).
  • Previous studies in animal models suggest that reducing CRP levels through extracorporeal apheresis can mitigate tissue damage and improve cardiac function post-AMI.
  • Elevated CRP levels are associated with various acute inflammatory diseases, necessitating effective therapeutic strategies.

Purpose of the Study:

  • To develop and evaluate a novel adsorber, PentraSorb CRP, for the selective removal of CRP from human plasma.
  • To assess the efficiency, specificity, and reusability of the PentraSorb CRP resin for clinical applications.
  • To determine the potential of CRP apheresis using PentraSorb CRP as a therapeutic approach for inflammatory diseases.

Main Methods:

  • Development of a novel resin-based adsorber (PentraSorb CRP) designed for specific CRP binding.
  • Evaluation of CRP depletion efficiency and selectivity using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
  • Testing of the adsorber's performance across a range of CRP concentrations (10-100 mg/L) and flow rates (17-40 mL/min), and assessment of resin regenerability and biocompatibility.

Main Results:

  • The PentraSorb CRP resin demonstrated highly specific binding of CRP from human plasma with minimal co-purification of other proteins.
  • Efficient and selective CRP depletion was achieved across various concentrations and flow rates, comparable to small-scale and larger adsorber volumes.
  • The resin maintained its CRP binding capacity and biocompatibility after up to 200 regeneration cycles.

Conclusions:

  • PentraSorb CRP is a novel, specific, and efficient resin for CRP apheresis.
  • The developed adsorber is suitable for clinical application in managing inflammatory conditions.
  • CRP apheresis holds potential as a therapeutic strategy for patients with acute myocardial infarction, stroke, acute pancreatitis, and Crohn's disease.

Keywords:
ApheresisC-reactive proteinImmunoadsorptionMyocardial infarction

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