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Virulence Characterization of Venturia inaequalis Reference Isolates on the Differential Set of Malus Hosts

Valérie Caffier1, Andrea Patocchi2, Pascale Expert1

  • 1INRA, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France; AgroCampus-Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, 49045 Angers, France; Université d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, 49045 Angers, France.

Plant Disease
|February 1, 2019
PubMed

Insights

Researchers characterized 23 Venturia inaequalis isolates on apple scab resistance genes. This work refines differential hosts for Rvi7 and establishes a reference set for studying host-pathogen interactions in the apple scab pathosystem.

Area of Science:

  • Plant Pathology
  • Genetics
  • Agricultural Science

Background:

  • Gene-for-gene (GfG) relationships are crucial for understanding plant-pathogen interactions.
  • Previous work identified 17 apple scab resistance genes but lacked a reference set of pathogen isolates.
  • A well-defined set of differential hosts and pathogen isolates is needed for the Venturia inaequalis-Malus pathosystem.

Purpose of the Study:

  • To improve the set of differential hosts for the apple scab resistance gene h(7).
  • To propose the apple genotype LPG3-29 as carrying the Rvi7 resistance gene.
  • To characterize a reference set of Venturia inaequalis isolates for their virulence profiles.

Main Methods:

  • Characterization of 23 V. inaequalis isolates on 14 differential apple hosts with known resistance genes.
  • Controlled condition testing to determine isolate virulence on specific resistance gene hosts.
  • Analysis of observed virulence patterns among the V. inaequalis isolates.

Main Results:

  • The apple genotype LPG3-29 was identified as carrying the Rvi7 resistance gene.
  • Sixteen distinct virulence patterns were observed among the 23 V. inaequalis isolates.
  • Isolates exhibited varying complexity of virulence, from one to six virulences.

Conclusions:

  • A reference set of 23 V. inaequalis isolates characterized for virulence has been established.
  • This resource will aid in identifying new apple scab resistance genes in apple germplasm.
  • Further characterization of GfG relationships will enhance understanding of V. inaequalis-Malus pathosystem interactions.

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