Related Experiment Video
Updated: Jan 30, 2026
11:32
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
Published on: December 4, 2010
15.8K
The UAP56-Interacting Export Factors UIEF1 and UIEF2 Function in mRNA Export.
Hans F Ehrnsberger1, Christina Pfaff1, Ines Hachani1
1Department of Cell Biology & Plant Biochemistry, Biochemistry Centre, University of Regensburg, D-93053 Regensburg, Germany.
Plant Physiology
|February 1, 2019
Summary
UAP56-interacting export factors 1 and 2 (UIEF1/2) function in plant mRNA export. They cooperate with ALY proteins to ensure efficient mRNA transport from the nucleus to the cytoplasm.
Area of Science:
- Molecular Biology
- Plant Science
- Cell Biology
Background:
- Nucleocytosolic mRNA transport is crucial for gene expression in eukaryotes.
- The specific mechanisms and factors involved in plant mRNA export are not well understood.
Purpose of the Study:
- To investigate the function of Arabidopsis UIEF1 and UIEF2 as potential mRNA export factors.
- To elucidate the role of UIEF proteins in plant mRNA export pathways.
Main Methods:
- Genetic analysis of UIEF1 and UIEF2 single and double mutants in Arabidopsis thaliana.
- Creation and analysis of a sextuple mutant combining uief and aly mutations.
- RNA binding assays with recombinant UIEF1.
- Analysis of polyadenylated mRNA distribution in different mutant lines.
Main Results:
- UIEF1 and UIEF2 interact with the RNA helicase UAP56.
- UIEF1 exhibits RNA-binding activity, primarily mediated by its C-terminal region.
- Mutations in UIEF1/2 cause modest growth defects, while combined mutations with aly factors lead to severe growth impairment and developmental defects.
- Nuclear mRNA accumulation is exacerbated in sextuple mutants compared to single or double mutants.
Conclusions:
- UIEF1 and UIEF2 are confirmed as plant mRNA export factors.
- UIEF proteins function in conjunction with ALY1-ALY4 to facilitate efficient nucleocytosolic mRNA transport.
- These findings contribute to understanding the molecular mechanisms of mRNA export in plants.
More Related Videos
Related Concept Videos
02:31Nuclear Export of mRNA
8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
02:31Nuclear Export of mRNA
5.4K
5.4K
02:19Export of Mitochondrial and Chloroplast Genes
4.2K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.2K
01:42Nuclear Export
5.0K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.0K
01:32Export of Misfolded Proteins out of the ER
5.3K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
5.3K
01:31ABC Transporters: Exporter
6.5K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.5K