Related Experiment Video

Updated: Jan 30, 2026

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
09:16

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Published on: February 28, 2018

8.1K

Isolation and characterization of patient-derived CNS metastasis-associated stromal cell lines

Ben Yi Tew1, Christophe Legendre2, Gerald C Gooden1,2

  • 1Department of Translational Genomics, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA.

Oncogene
|February 1, 2019
PubMed

Insights

Researchers isolated and characterized CNS metastasis-associated stromal cells (cMASCs) from human tumors. These cMASCs showed tumor-inhibitory functions, restricting growth and inducing desmoplasia in vivo.

Area of Science:

  • Oncology
  • Cancer Biology
  • Tumor Microenvironment Research

Background:

  • The role of stromal cells within the tumor microenvironment of central nervous system (CNS) metastases is not well understood.
  • Stromal cells are crucial components of the tumor microenvironment, influencing tumor progression and treatment response.

Purpose of the Study:

  • To isolate and characterize human-derived stromal cells from CNS metastases (CM).
  • To investigate the functional role of these stromal cells in the context of CM.

Main Methods:

  • Generation of patient-derived cell lines (PDCs) from breast or lung CM.
  • Analysis of PDCs using DNA/RNA sequencing, DNA methylation profiling, and immunophenotypic assays.
  • In vivo functional analysis through co-implantation of stromal PDCs (termed cMASCs) with tumorigenic cells in mice.

Main Results:

  • cMASCs exhibited normal genotypes and molecular profiles resembling each other, suggesting a common origin.
  • Gene expression signatures indicated associations with cancer-associated fibroblasts (CAFs), epithelial-to-mesenchymal transition, and mesenchymal stem cells, with high collagen expression.
  • Functional assays revealed that cMASCs restricted tumor growth and induced desmoplasia in vivo.

Conclusions:

  • Human-derived CNS metastasis-associated stromal cells (cMASCs) were successfully isolated and characterized.
  • cMASCs possess tumor-inhibitory functions, potentially contributing to a protective host response against tumor growth in CNS metastases.
  • These findings highlight a novel stromal cell population with therapeutic potential in managing CNS metastases.

More Related Videos

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta07:06

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Published on: April 3, 2017

48.8K
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling11:26

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Published on: January 18, 2015

17.2K

Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.3K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.6K
CNS Depressants: Alcohol and Nicotine01:27

CNS Depressants: Alcohol and Nicotine

Ethanol, a clear colorless alcohol, has been consumed by humans for millennia, but its effects on the body are far from benign. At lower doses, it induces decreased inhibitions and loquaciousness, leading to its social appeal. However, it can cause severe consequences at higher doses, such as coma and respiratory depression, due to its zero-order elimination kinetics. Chronic ethanol abuse wreaks havoc on multiple organ systems, particularly the CNS and the liver. Abrupt cessation of ethanol...
1.2K
CNS Depressants: Barbiturates and Benzodiazepines01:14

CNS Depressants: Barbiturates and Benzodiazepines

CNS depressants include drugs from the category of barbiturates and benzodiazepines. They are valuable medications for managing anxiety disorders and insomnia. Barbiturates, once used to induce and maintain sleep, have been replaced mainly by benzodiazepines due to barbiturate's toxicity, tolerance, and overdose risks. They interact with GABAA receptors, leading to sedation at low doses and potentially coma and death at higher doses. Phenobarbital, a long-acting barbiturate, possesses...
1.3K
CNS Stimulants: Psychedelic Agents01:22

CNS Stimulants: Psychedelic Agents

Hallucinogens, also known as psychedelic drugs, are a class of substances known for their ability to alter perception, cognition, and emotions. Despite their profound effects on the mind, these drugs are non-addictive, setting them apart from many other abused substances. The mechanism of action of these drugs lies in their impact on the 5-HT2A receptor in the brain. Upon activation, this receptor couples to Gq-type G proteins, triggering a cascade that releases intracellular calcium. This...
789
Lossless Lines01:23

Lossless Lines

In electrical engineering, a lossless transmission line is characterized by a purely imaginary propagation constant and a resistive characteristic impedance. The ABCD parameters, which describe the relationship between the input and output voltages and currents, indicate an equivalent π circuit with an imaginary series impedance and a shunt admittance. This results in a transmission line that, when the product of the phase constant (beta) and the length of the line is less than pi, exhibits...
565