Related Experiment Video
Updated: Jan 30, 2026

Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Comparison Study of Three Hydroxyapatite-Based Bone Substitutes in a Calvarial Defect Model in Rabbits
Purpose:
The aim of this study was to investigate the in vivo performance, including bone regeneration and biomaterial degradation, of three hydroxyapatite-based bone substitutes at surgically created defects in the cranium of rabbits.
Materials And Methods:
A bovine-derived hydroxyapatite (BHA), a synthetic hydroxyapatite (SHA), and a nano-hydroxyapatite with collagen I (nHAC) composite were characterized and then placed in calvarial defects in rabbits. After surgery at 6 weeks and 12 weeks, the animals were sacrificed for further microcomputed tomography, histologic, and histomorphometric analyses. The amounts of newly formed bone, residual grafting materials, connective tissues, and tartrate-resistant acid phosphatase staining positive multinucleate cells were evaluated.
Results:
The three hydroxyapatite-based bone substitutes displayed different surface topography. BHA had the highest amount of newly formed bone at both 6 weeks and 12 weeks, yet no significant difference was found between the three groups at 6 weeks. The amount of residual materials was significantly higher in the SHA group than the other groups at both time points. The amount of connective tissue was highest in BHA. More multinucleated cells were observed in the nHAC group.
Conclusion:
The three tested bone substitutes are suitable for grafting defects related to early bone healing. BHA had a better impact on bone formation with the prolongation of time. An integration of collagen type I and nano-hydroxyapatite may not improve bone regeneration of pure synthetic hydroxyapatite but helps to modify the degradation rate of pure hydroxyapatite.
More Related Videos
11:31Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Published on: October 31, 2012
04:17Author Spotlight: Development and Evaluation of a Standardized Rat Model for Calvarial Suture-Bony Composite Defects
Published on: May 10, 2024
Related Concept Videos
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Pharmacokinetic Models: Comparison and Selection Criterion
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.
Trigonometric Substitution
Rationalizing Substitutions
Lumber Defects
Shakes are minor fractures that run along or across the wood's annual rings, while wane is...
Multiple Comparison Tests
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...