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[Serological and molecular genetic analysis of a family with B(A) blood type]
Wenjing Zhang1, Shouyong Hun, Yuxia Wang
1Institute of Hematology, Blood Center of Shandong Province, Jinan, Shandong 250014, China. yunlongzhuang@gmail.com.
Objective:
To analyze the blood type of a family with B(A) blood type.
Methods:
The serological blood type of the family was determined by routine tube method. Exons 6 and 7 of the ABO gene were amplified by PCR and subjected to Sanger sequencing.
Results:
Serological testing of the proband and her elder son showed a discrepancy which was initially identified as B(A) subtype. Her husband and second son were identified as blood type O. Sequencing of the proband and her elder son has identified an O02 allele and a 640A>G mutation compared with the B101 gene. Her husband and second son possessed the same genotype of O01/O02.
Conclusion:
The 640A>G mutation of ABO gene probably underlies the B(A)04 subtype.
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