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High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

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Functionalized Polymeric Materials with Bio-Derived Antimicrobial Peptides for "Active" Packaging.

Bruna Agrillo¹, Marco Balestrieri², Marta Gogliettino³

¹Materias S.r.l., Corso N. Protopisani, 80146 Napoli, Italy. bruna.agrillo@ibbr.cnr.it.

International Journal of Molecular Sciences

|February 2, 2019

Summary

Antimicrobial food packaging using peptide-functionalized Polyethylene Terephthalate (PET) significantly reduces bacteria, yeast, and mold. This active packaging enhances food safety and extends shelf-life by inhibiting microbial growth and biofilm formation.

Keywords:

active packaging antimicrobial peptides food shelf-life foodborne pathogens plastic materials

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Area of Science:

Food Science and Technology
Materials Science
Biotechnology

Background:

Food packaging requires advanced materials to maintain quality, safety, and shelf-life.
Antimicrobial packaging is a key innovation with significant socio-economic implications.
Polymer functionalization offers novel strategies for active packaging development.

Purpose of the Study:

To develop and evaluate an active food packaging system using peptide-functionalized Polyethylene Terephthalate (PET).
To assess the antimicrobial and antibiofilm efficacy of the functionalized PET against foodborne microorganisms.
To determine the stability and reusability of the active packaging material.

Main Methods:

Plasma activation of Polyethylene Terephthalate (PET) surfaces.
Covalent bio-conjugation of a synthetic peptide (1018K6) to the activated PET surface.
Evaluation of antimicrobial activity against total bacterial count, yeast, and mold in food-dairy products.
Assessment of antibiofilm efficacy against *Listeria monocytogenes*.

Main Results:

High yield immobilization of the peptide onto PET with no observed release under various conditions.
Significant reduction in total bacterial count, yeast, and mold spoilage in food-dairy products.
Enhanced inhibition of biofilm formation by *Listeria monocytogenes* compared to unmodified PET.

Conclusions:

Peptide-functionalized PET serves as effective active antimicrobial packaging.
This technology demonstrates potential for reducing pathogen development and extending food shelf-life.
The material exhibits chemical stability and reusability, offering practical advantages for the food industry.