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RECK isoforms differentially regulate fibroblast migration by modulating tubulin post-translational modifications

Ha Neul Lee1, Oye A Bosompra2, Hilary A Coller3

  • 1Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Biochemical and Biophysical Research Communications
|February 2, 2019
PubMed

Insights

RECK isoforms regulate cell migration by altering α-tubulin post-translational modifications (PTMs). These RECK-mediated changes in tubulin acetylation and detyrosination impact fibroblast migration rates and integrin-extracellular matrix interactions.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell migration is crucial for development and immunity.
  • RECK isoforms influence invasion by interacting with MMP9.
  • Previous work identified RECK isoform-mediated invasion pathways.

Purpose of the Study:

  • To elucidate the mechanism by which RECK isoforms affect cell migration.
  • To investigate the role of α-tubulin post-translational modifications (PTMs) in RECK-mediated cell migration.
  • To identify upstream regulators of RECK's effects on tubulin PTMs.

Main Methods:

  • Analysis of RECK isoform effects on α-tubulin acetylation and detyrosination.
  • Assessment of fibroblast migration rates under varying RECK isoform conditions.
  • Investigation of MMP9 and integrin activity in regulating tubulin PTMs.

Main Results:

  • Both canonical and short RECK isoforms modulate tubulin acetylation and detyrosination levels.
  • Altered tubulin PTMs are sufficient to modulate fibroblast migration rates.
  • MMP9 and integrin activity were identified as upstream regulators of tubulin acetylation and detyrosination.

Conclusions:

  • RECK isoforms impact cell migration through modulation of α-tubulin PTMs.
  • RECK isoforms exert opposing effects on cell migration via MMP9-dependent regulation of integrin-ECM interactions and microtubule PTMs.
  • This study defines a molecular pathway linking RECK, MMP9, integrin activity, and tubulin PTMs in regulating cell migration.
Keywords:
Fibroblast migrationIntegrinMMP9RECK isoformsTubulin PTM

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