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Updated: Jan 30, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
Separation of polystyrene nanoparticles with different coatings using two-dimensional off-line coupling of
Zengchao You1, Norbert Jakubowski2, Ulrich Panne1
1Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Str. 11, D-12489 Berlin, Germany; Humboldt-Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, D-12489 Berlin, Germany.
Abstract:
The successful off-line coupling of asymmetrical flow field flow fractionation (AF4) and capillary electrophoresis (CE) for separation of nanoparticles (NPs) with different surface coatings was shown. We could successfully demonstrate that, in a certain NP size range, hyphenation of both techniques significantly improved the separation of differently coated NPs. Three mixtures of polystyrene nanoparticles (PS-NPs) with comparable core sizes but different coatings (no coating/carboxyl-coated) were studied. Separation in either method resulted in non-baseline resolved or non-separated peaks. In contrast, two-dimensional off-line coupling of AF4 and CE resulted in clearly separated regions in their 2 D plots in case of 20 and 50 nm particle mixtures, whereas the 100 nm NP mixture could not be separated at all. Various factors affecting the separation like hydrodynamic diameter or SDS concentration were discussed.
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