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Related Experiment Video

Updated: Jan 30, 2026

Electroporation of Mycobacteria

Electroporation of Mycobacteria

Published on: May 23, 2008

Alanine dehydrogenases in mycobacteria.

Ji-A Jeong¹, Jeong-Il Oh²

¹Department of Integrated Biological Science, Pusan National University, Busan, 46241, Republic of Korea.

Journal of Microbiology (Seoul, Korea)

|February 2, 2019

Summary

L-alanine dehydrogenase (Ald) is crucial for Mycobacterium tuberculosis survival under stress by managing nutrient and energy balance. Ald inhibitors show potential when combined with existing tuberculosis drugs.

Keywords:

Lrp/AsnC family regulator alanine dehydrogenase gene regulation mycobacteria redox homeostasis

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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

Area of Science:

Biochemistry
Microbiology
Enzymology

Background:

L-alanine dehydrogenase (Ald) is a key antigen in Mycobacterium tuberculosis.
Ald catalyzes the pyruvate-to-alanine conversion, impacting NADH/NAD+ homeostasis.
Ald gene expression is upregulated by alanine and under respiration-inhibitory conditions.

Purpose of the Study:

To investigate the role of Ald in mycobacterial survival.
To understand the regulation of Ald expression.
To explore Ald as a therapeutic target for tuberculosis.

Main Methods:

Gene expression analysis in Mycobacterium tuberculosis and Mycobacterium smegmatis.
Biochemical characterization of Ald enzyme activity.
Investigated AldR transcriptional regulator function.

Main Results:

Ald expression is upregulated by alanine and stress conditions (oxygen limitation, nutrient starvation).
AldR mediates the upregulation of ald genes.
Mycobacterial Alds are essential for alanine utilization and survival under stress.
Ald inhibitors have been developed and show potential in combination therapy.

Conclusions:

Ald plays a vital role in mycobacterial adaptation and survival.
AldR is a key regulator of Ald expression.
Ald is a promising target for novel anti-tubercular strategies, particularly in combination therapy.