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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
06:14

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

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Construction of Oncolytic Herpes Simplex Virus with Therapeutic Genes of Interest

Andranik Kahramanian1, Toshihiko Kuroda1, Hiroaki Wakimoto2

  • 1Department of Neurosurgery, Brain Tumor Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2019
PubMed

Insights

Oncolytic herpes simplex virus (oHSV) shows promise for treating cancers like glioblastoma. Researchers developed a method using bacterial artificial chromosomes to engineer oHSV with therapeutic genes, enhancing its anti-cancer potential.

Area of Science:

  • Oncolytic virotherapy
  • Cancer gene therapy
  • Molecular virology

Background:

  • Herpes simplex virus (HSV) is a well-researched platform for oncolytic virotherapy.
  • The FDA approval of talimogene laherparepvec (T-VEC) has spurred interest in oncolytic HSV (oHSV) for treating difficult cancers, including glioblastoma.
  • HSV's large genome facilitates the incorporation of therapeutic transgenes to boost anticancer activity.

Purpose of the Study:

  • To describe a bacterial artificial chromosome (BAC)-based method for constructing oHSV.
  • To demonstrate the creation of recombinant oHSV vectors expressing transgenes for therapeutic or reporter purposes.

Main Methods:

  • Utilized a bacterial artificial chromosome (BAC) system for oHSV genome manipulation.
  • Engineered oHSV to express specific transgenes by incorporating them into the viral genome.
  • Routinely applied this method in the laboratory for oHSV construction.

Main Results:

  • Successfully developed and implemented a BAC-based method for oHSV construction.
  • Created multiple recombinant oHSV vectors carrying different therapeutic or reporter genes.
  • Established a reproducible laboratory workflow for oHSV engineering.

Conclusions:

  • The described BAC-based method provides a robust platform for generating engineered oHSV.
  • This approach facilitates the development of novel oHSV therapies with enhanced anticancer efficacy.
  • The method is adaptable for creating oHSV expressing various transgenes for diverse research and therapeutic applications.
Keywords:
Bacterial artificial chromosomeCre recombinationFlp recombinationGlioblastomaHerpes simplex virus (HSV)

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