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Updated: Jan 30, 2026

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
Published on: January 26, 2017
Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes
Guillaume Adelmant1,2, Brijesh K Garg1, Maria Tavares1
1Department of Cancer Biology and Blais Proteomics Center, Dana-Farber Cancer Institute, Boston, Massachusetts.
Abstract:
Affinity purification followed by mass spectrometry has become the technique of choice to identify binding partners in biochemical complexes isolated from a physiologic cellular context. In this report we detail our protocol for tandem affinity purification (TAP) primarily based on the use of the FLAG and HA peptide epitopes, with a particular emphasis on factors affecting yield and specificity, as well as steps to implement an automated version of the TAP procedure. © 2019 by John Wiley & Sons, Inc.
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