Sensitive Water-Soluble Fluorescent Probe Based on Umpolung and Aggregation-Induced Emission Strategies for Selective...

Using Hg²⁺-induced umpolung reaction and aggregation induced emission (AIE), we have rationally developed a water-soluble fluorescent probe 2,2'-(((4-(4,5-bis(4-methoxyphenyl)-1-phenyl-1H-imidazol-2-yl)phenyl)methylene)bis(sulfanediyl))diethanol (MPIPBS) for Hg²⁺ detection. MPIPBS was found to have high selectivity and sensitivity toward Hg²⁺ detection. The mechanism of MPIPBS response to Hg²⁺ was verified by ¹H NMR titration, HPLC, and HRMS spectroscopy. The detection limit was examined to be 1.45 nM, which is lower than most reported probes for Hg²⁺. Taking advantage of excellent optical properties of AIEgen, a paper based sensor for Hg²⁺ detection was fabricated by immobilizing the MPIPBS on Waterman test paper. Meanwhile, MPIPBS showed satisfactory analytical performance in real water and urine samples. Further, thanks to the high water solubility, cell membrane permeability and low cytotoxicity, MPIPBS was further used to detect Hg²⁺ both in living cells and zebrafish. We anticipate that the prepared probe was available to detect Hg²⁺ in environment and biosamples.