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Immuno-electron microscopy of chondrocyte-derived cells in the rheumatoid cartilage-pannus junction

K D Muirden1, S A Allard, K Rogers

  • 1Department of Medicine, Royal Melbourne Hospital, Victoria, Australia.

Rheumatology International
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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.