Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Design Consideration01:22

Design Consideration

571
Designing a structure involves a series of considerations, primarily the material's ultimate strength, calculated through tests that measure changes under increased force until the material reaches its breaking point or limit. The ultimate load, where the material breaks, is divided by its original cross-sectional area, resulting in the ultimate normal stress or strength. The ultimate shearing stress is another significant factor taken into account.
The factor of safety is another key...
571
Tensile Strength Considerations of Concrete01:16

Tensile Strength Considerations of Concrete

546
Considering the tensile strength of concrete involves recognizing that the theoretical strength of cement paste can be up to a thousand times higher than what is observed in practical applications. This significant discrepancy is largely attributed to the presence of microscopic cracks within the concrete. These cracks tend to amplify stress at their tips when a load is applied, a phenomenon explained by Griffith's theory of brittle fracture.
The dimensions and shape of a concrete specimen...
546
Transmission Line Design Considerations01:23

Transmission Line Design Considerations

629
Aluminum has become the material of choice for overhead transmission lines, surpassing copper due to its abundance and cost-effectiveness. The most prevalent type is the aluminum conductor, steel-reinforced (ACSR), which combines aluminum strands around a steel core. Other variants include all-aluminum conductors (AAC), all-aluminum alloy conductors (AAAC), aluminum conductor alloy-reinforced (ACAR), and aluminum-clad steel conductors. Advanced designs, such as aluminum conductors with steel...
629
Special considerations while measuring pulse01:13

Special considerations while measuring pulse

955
Assessing a patient's pulse is a fundamental skill in healthcare, but certain situations require special attention:
955
Energy Considerations in Open Channel Flow01:27

Energy Considerations in Open Channel Flow

596
Open channel flow, where a fluid flows with a free surface exposed to the atmosphere, is primarily governed by gravitational and surface effects, distinguishing it from closed conduit or pipe flow. In open channels such as rivers, canals, and artificial channels, energy analysis provides valuable insights into flow behavior and the relationship between depth, velocity, and slope.Specific Energy and Flow DepthIn open channel flow, the specific energy, E, combines the gravitational potential...
596
Neural Regulation01:37

Neural Regulation

43.3K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
43.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-nucleus transcriptomics resolves multiple fate dynamics between inflorescence meristem and primary stem.

Science advances·2026
Same author

Cell geometry and mechanical stress coordinate stomatal division orientation.

Cell reports·2026
Same author

Long-term <i>in toto</i> cell tracking using lightsheet microscopy of the zebrafish tailbud.

Wellcome open research·2026
Same author

Extracellular space diffusion modelling identifies distinct functional advantages of glutamatergic and GABAergic synapse geometries.

Fluids and barriers of the CNS·2026
Same author

Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries.

bioRxiv : the preprint server for biology·2025
Same author

Picrotoxin-Induced Epileptogenic Hippocampal Organotypic Slice Cultures (hOTCs).

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Jan 30, 2026

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy
11:00

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Published on: April 9, 2018

14.6K

Considerations for Imaging and Analyzing Neural Structures by STED Microscopy.

Martin O Lenz1, Jan Tønnesen2,3

  • 1Cambridge Advanced Imaging Centre, University of Cambridge, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2019
PubMed
Summary
This summary is machine-generated.

This chapter details key parameters for acquiring and analyzing Stimulated Emission Depletion (STED) microscopy images. Proper methods ensure accurate extraction of geometric and morphological data from brain tissue cellular structures.

Keywords:
Dendritic spinesFluorescence microscopyImage analysisLive cell imagingSTEDSuper-resolution

More Related Videos

Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

4.9K
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
14:11

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Published on: May 4, 2014

16.4K

Related Experiment Videos

Last Updated: Jan 30, 2026

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy
11:00

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Published on: April 9, 2018

14.6K
Author Spotlight: Decoding Mitochondrial Aging
08:48

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

4.9K
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
14:11

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Published on: May 4, 2014

16.4K

Area of Science:

  • Neuroscience
  • Microscopy
  • Cell Biology

Background:

  • Stimulated Emission Depletion (STED) microscopy offers high-resolution imaging of cellular structures in brain tissue.
  • Extracting detailed morphological data from STED images requires careful consideration of acquisition and analysis parameters.

Purpose of the Study:

  • To highlight critical parameters for acquiring and analyzing STED microscopy images.
  • To guide the interpretation of STED microscopy data for cellular morphology.

Main Methods:

  • Focus on image acquisition parameters relevant to STED microscopy.
  • Discuss essential image analysis techniques for morphological data extraction.
  • Emphasize considerations for interpreting STED data.

Main Results:

  • Identification of key parameters influencing STED image quality and data integrity.
  • Establishment of best practices for STED image acquisition and analysis.
  • Guidelines for reliable morphological assessment of neuronal structures.

Conclusions:

  • Optimized STED microscopy acquisition and analysis are crucial for accurate cellular morphology studies.
  • Adherence to highlighted parameters ensures credible geometric and morphological data extraction.
  • This chapter provides a framework for maximizing the utility of STED microscopy in neuroscience research.