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Membrane androgen receptors (OXER1, GPRC6A AND ZIP9) in prostate and breast cancer: A comparative study of their

Konstantina Kalyvianaki1, Athanasios A Panagiotopoulos1, Panagiotis Malamos1

  • 1Laboratory of Experimental Endocrinology, University of Crete, School of Medicine, Heraklion 71013, Greece.

Steroids
|February 2, 2019
PubMed

Insights

This study compares three membrane androgen receptors (mARs) – OXER1, GPRC6A, and ZIP9 – in prostate and breast cancers. Findings reveal distinct expression patterns, aiding understanding of nonclassical androgen actions in cancer progression.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Endocrinology

Background:

  • Androgens regulate cellular processes via membrane-initiated actions, distinct from nuclear receptors.
  • Novel membrane androgen receptors (mARs) mediate these nonclassical, rapid androgen effects.
  • OXER1, GPRC6A, and ZIP9 are identified as potential mARs involved in cancer.

Purpose of the Study:

  • To comparatively analyze OXER1, GPRC6A, and ZIP9 as mARs in prostate and breast cancer.
  • To investigate their in silico pharmacology, gene expression, and immunocytochemical profiles.
  • To determine their expression patterns and intracellular distribution in human tumor tissues.

Main Methods:

  • In silico pharmacology analysis.
  • Gene expression profiling.
  • Immunocytochemistry and immunohistochemistry on cell lines and human tissue specimens.

Main Results:

  • Comparative analysis of OXER1, GPRC6A, and ZIP9 expression and distribution in cancer cell lines.
  • Immunohistochemical evaluation of these mARs in human prostate and breast tumors versus non-tumoral tissues.
  • Identification of specific expression patterns and subcellular localization for each receptor.

Conclusions:

  • OXER1, GPRC6A, and ZIP9 exhibit distinct expression profiles in prostate and breast cancers.
  • These mARs show varied intracellular localization, suggesting diverse functional roles.
  • Understanding these mARs is crucial for targeting nonclassical androgen signaling in cancer therapy.
Keywords:
AndrogensBreast cancerGPCRMembrane receptorsOXER1Prostate cancer

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