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Updated: Jan 30, 2026

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
Published on: July 3, 2017
Principles of Stress Granules Revealed by Imaging Approaches
Briana Van Treeck1, Roy Parker1,2
1Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80303.
Abstract:
Eukaryotic cells contain a large number of RNA-protein assemblies, generically referred to as ribonucleoprotein (RNP) granules. Such RNP granules include stress granules and P-bodies in the cytosol and the nucleolus, Cajal bodies, and paraspeckles in the nucleus. A variety of imaging approaches have been used to reveal different components, structural features, and dynamics of RNP granules. In this review, we discuss imaging approaches that have been used to study stress granules and the insights gained from these experiments. A general theme is that these approaches can be transferred to other RNP granules to examine similar aspects of their composition, ultrastructure, dynamics and control.
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