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Cancer cell-expressed SLAMF7 is not required for CD47-mediated phagocytosis

Yuan He1, Renee Bouwstra1, Valerie R Wiersma1

  • 1Department of Hematology, University of Groningen, University Medical Center Groningen (UMCG), Groningen, GZ, 9713, The Netherlands.

Nature Communications
|February 3, 2019
PubMed

Insights

SLAMF7 expression on cancer cells is not required for CD47 antibody therapy or phagocytosis. CD47 expression, however, negatively impacts patient survival, making it a better target for cancer immunotherapy selection criteria.

Area of Science:

  • Immunology
  • Oncology
  • Translational Medicine

Background:

  • CD47 is a key target in cancer immunotherapy, with antibodies in clinical trials.
  • Identifying patient selection criteria for CD47-targeted therapy is crucial.
  • SLAMF7 expression was previously suggested as a requirement for CD47 antibody-mediated phagocytosis.

Purpose of the Study:

  • To investigate the role of SLAMF7 expression on cancer cells in CD47 antibody therapy.
  • To determine if SLAMF7 expression impacts phagocytosis induced by CD47 or CD20 antibodies.
  • To assess the prognostic value of SLAMF7 and CD47 expression in Diffuse Large B-Cell Lymphoma (DLBCL).

Main Methods:

  • In vitro experiments assessing phagocytosis.
  • Analysis of SLAMF7 and CD47 expression in cancer cells and patient samples.
  • Correlation analysis with patient survival data.

Main Results:

  • SLAMF7 expression on cancer cells is not required for CD47 antibody-mediated phagocytosis.
  • SLAMF7 does not influence phagocytosis induced by rituximab (CD20 antibody).
  • CD47 expression is associated with poorer overall and progression-free survival in DLBCL patients.

Conclusions:

  • Cancer cell SLAMF7 expression is not a prerequisite for phagocytosis and should not be used for patient selection in CD47-targeted therapies.
  • CD47 expression, unlike SLAMF7, is a significant negative prognostic factor in DLBCL.
  • CD47 expression warrants further investigation as a biomarker for CD47-targeted cancer immunotherapy.

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