Measuring Calumenin Impact on ER-Calcium Depletion Using Transient Calumenin Overexpression and Silencing
Réginald Philippe1, Olivier Mignen2
1Institut National de la Santé et de la Recherche Médicale, U1151, Paris, France.
Abstract:
Calumenin is a secretory pathway protein regulating different endoplasmic reticulum (ER) proteins such as the sarco-endoplasmic reticulum calcium ATPase (SERCA) pumps. Combined with its diverse cellular distribution, its calcium-binding ability, and its interaction with proteins involved in calcium signaling, it is easy to speculate on future description of important roles of calumenin in calcium homeostasis in many cell types, as it was initially observed in muscle cells. In this chapter, we describe basic techniques to modulate calumenin expression and detect its impact on ER calcium content using classic transfection and Western blot techniques, as well as ER calcium measurement using microplate reader.
Related Concept Videos
Impact of Groups on Groups
MOSFET: Depletion Mode
The primary characteristic of depletion-mode MOSFETs is their ability to conduct current between the drain and source terminals without gate bias. This inherent conductivity...
Series R—L Circuit Transients
Using Kirchhoff's Voltage Law (KVL) to analyze this circuit helps determine the total asymmetrical fault current, which consists...
Impact
When particles with different initial velocities collide, they induce deformation by applying equal and opposite impulses. At the point of maximum deformation, the particles move together with...
Transient and Steady-state Response
These test signals are integral in designing control systems to exhibit two key performance aspects: transient response and steady-state...
Impact of Groups on Individuals


