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Published on: July 5, 2017
Adsorption isotherm studies on the interaction between polyphenols and apple cell walls: Effects of variety, heating
Dongjie Liu1, Patricia Lopez-Sanchez2, Marta Martinez-Sanz3
1Key Laboratory of Plant Cell Walls & Plant Resistance, Molecular Analysis & Genetic Improvement Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
Abstract:
The adsorption capacity of principal phenolic compounds onto cell walls from three apple varieties was investigated. Isothermal adsorption modelled with Langmuir, Freundlich and Redlich-Peterson equations were carried out over a range of concentrations from 0.5 to 30 mM before and after cell walls were subjected to boiling, oven-drying or freeze-drying. The isotherm data were best fitted by the Langmuir model in all cases. Polyphenols selectively adsorbed onto cell walls with maximum binding capacities ranging from 140 to 580 µg/mg cell walls depending on surface charge. Increased pectin in apple cell walls caused a 129%-311% decrease in the adsorption of negatively charged polyphenols, presumably due to electrostatic repulsive forces. Boiling had limited effect on cell wall polysaccharides and polyphenol-cell wall interactions. However, more than twofold reduction in binding capacities of polyphenols was induced after drying by altering the structural (i.e. binding sites) and compositional (i.e. pectin degradation) characteristics of cell walls.
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