Histologic characteristics of the subscapularis tendon from muscle to bone: reference to subscapularis lesions

Although subscapularis tendon lesions seem to differ from those of the supraspinatus tendon, the features they have in common suggest that the subscapularis tendon may also have 2 distinct layers. Our aim was therefore to characterize the histologic structure of the subscapularis tendon from its humeral insertion point to the musculotendinous junction.

A histologic study was performed on 10 autopsy samples. The subscapularis tendon was extracted in one piece from the musculotendinous junction to the humeral insertion point and was prepared using standard (hematoxylin-eosin-saffron) staining.

Histologic analysis revealed 2 fibrous layers, distinguishable by the orientation of the collagen bundles. The deep layer was thinner and composed of parallel longitudinal collagen fibers inserting onto the lesser tuberosity flush with the cartilage. The superficial layer was thicker and composed of interdigitated collagen bundles inserting onto the lesser and the greater tuberosity after splitting into 2 bands, 1 lining the floor of the bicipital groove, and the other extending over the long head of the biceps tendon across the groove. Each layer formed an independent musculotendinous junction in the subscapularis muscle.

The subscapularis tendon is composed of 2 distinct fibrous layers, just like the supraspinatus tendon, but arranged differently. The superficial layer of the subscapularis tendon passes across the bicipital groove and forms a fibrous ring around the long head of the biceps tendon that stabilizes the latter in the bicipital groove. These results explain some of the specific features of subscapularis tears described in the literature, namely, delamination and biceps subluxation.