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Updated: Jan 29, 2026
Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model
Published on: June 4, 2017
Basic Gene Expression Characteristics of Glioma Stem Cells and Human Glioblastoma
Daisuke Sakamoto1, Toshinori Takagi1, Mitsugu Fujita2
1Department of Neurosurgery, Hyogo College of Medicine, Hyogo, Japan.
Background:
Glioma stem cells (GSCs) play important roles in the tumorigenesis of glioblastoma multiforme (GBM). Using a novel cellular bioinformatics pipeline, we aimed to characterize the differences in gene-expression profiles among GSCs, U251 (glioma cell line), and a human GBM tissue sample.
Materials And Methods:
Total RNA was extracted from GSCs, U251 and GBM and microarray analysis was performed; the data were then applied to the bioinformatics pipeline consisting of a principal component analysis (PCA) with factor loadings, an intracellular pathway analysis, and an immunopathway analysis.
Results:
The PCA clearly distinguished the three groups. The factor loadings of the PCA suggested that v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN), dipeptidyl-peptidase 4 (DPP4), and macrophage migration-inhibitory factor (MIF) contribute to the stemness of GSCs. The intracellular pathway and immunopathway analyses provided relevant information about the functions of representative genes in GSCs.
Conclusion:
The newly-developed cellular bioinformatics pipeline was a useful method to clarify the similarities and differences among samples.
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