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Extracellular L-asparaginase production in solid-state fermentation by using sugarcane bagasse as support material
J J Muso Cachumba1, R Terán Hilares1, L P Brumano1
1a Department of Biotechnology, Engineering School of Lorena , University of São Paulo , Lorena , São Paulo , Brazil.
Preparative Biochemistry & Biotechnology
|February 5, 2019
Summary
This study optimized L-asparaginase production using sugarcane bagasse in solid-state fermentation. The findings demonstrate a viable method for large-scale enzyme manufacturing.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Industrial Microbiology
Background:
- L-asparaginase is a crucial enzyme in pharmaceuticals and food processing.
- Microbial production of L-asparaginase utilizes cost-effective feedstocks.
- Sugarcane bagasse (SCB) offers potential as a support for solid-state fermentation (SSF).
Purpose of the Study:
- To optimize L-asparaginase production by Aspergillus terreus using SCB in SSF.
- To evaluate the impact of carbon and nitrogen sources on enzyme yield.
- To assess the scalability of enzyme production in a column reactor.
Main Methods:
- Solid-state fermentation (SSF) using Aspergillus terreus.
- Optimization of carbon (starch, maltose) and nitrogen (asparagine, glutamine) sources via experimental design.
- Enzyme activity assay (U/L).
- Production in Erlenmeyer flasks and a horizontal column reactor.
Main Results:
- Optimal medium composition determined: 0.54% starch, 0% maltose, 0.44% asparagine, 1.14% glutamine.
- Achieved enzyme activity of 120.723 U/L in Erlenmeyer flasks.
- Demonstrated enzyme activity of 105.3 U/L in a SCB-packed column reactor.
Conclusions:
- Sugarcane bagasse is an effective support for extracellular L-asparaginase production via SSF.
- The column reactor system shows potential for large-scale L-asparaginase manufacturing.
- Optimized fermentation conditions enhance enzyme yield for industrial applications.
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