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Shift Work Disrupts Circadian Regulation of the Transcriptome in Hospital Nurses.

David Resuehr1, Gang Wu2, Russell L Johnson3

  • 1Department of Cellular, Integrative & Developmental Biology.

Journal of Biological Rhythms
|February 5, 2019
PubMed
Summary

Shift work disrupts circadian rhythms and causes genome-wide gene expression changes in nurses. Core body temperature and hormone rhythms desynchronize, impacting cellular pathways, even with similar sleep schedules.

Keywords:
chronobiologycircadian disruptionclock genesendocrinologymicroarraysleep

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Area of Science:

  • Chronobiology
  • Molecular Biology
  • Occupational Health

Background:

  • Circadian misalignment from shift work is linked to health issues.
  • Shift work's impact on genome-wide circadian regulation is not fully understood.

Purpose of the Study:

  • To investigate how shift work affects circadian rhythmicity and gene expression in hospital nurses.
  • To identify specific molecular pathways altered by night shift work.

Main Methods:

  • Continuous monitoring of locomotor activity and core body temperature (CBT) in day and night shift nurses.
  • Assay of cortisol and melatonin, and RNA extraction from peripheral blood mononuclear cells (PBMCs) every 3 hours.
  • Genome-wide microarray analysis of PBMCs to compare gene expression patterns between shift groups.

Main Results:

  • Night shift nurses showed phase desynchrony in CBT, cortisol, and melatonin rhythms compared to day shift nurses.
  • Distinct gene expression patterns were observed between day and night shift nurses, with differing pathway enrichments.
  • The number of rhythmic transcripts varied significantly among subjects, irrespective of shift type.

Conclusions:

  • Shift work significantly impacts circadian alignment and genome-wide gene expression in nurses' PBMCs.
  • Specific cellular pathways related to transcription, signal transduction, and the extracellular matrix are differentially regulated by shift work.
  • Core body temperature rhythm amplitude may be a more robust indicator of circadian rhythmicity than melatonin in shift workers.