Related Experiment Video

Updated: Jan 29, 2026

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

14.2K

SPSB1 enhances ovarian cancer cell survival by destabilizing p21

Hyun-Jung Kim1, Hye Jin Kim2, Mi-Kyung Kim2

  • 1Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Seoul, South Korea; Innovative Research Center for Control and Prevention of Women's Cancer, Ewha Womans University Mokdong Hospital, Seoul, South Korea.

Biochemical and Biophysical Research Communications
|February 5, 2019
PubMed

Insights

SPRY domain-containing SOCS box protein 1 (SPSB1) impacts ovarian cancer cell growth and survival. Knockdown of SPSB1 reduces viability and migration, while increasing apoptosis by stabilizing p21.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein.
  • The function of SPSB1 in cancer cells remains largely unknown.

Purpose of the Study:

  • To investigate the role and mechanism of SPSB1 in ovarian cancer cells.

Main Methods:

  • SPSB1 knockdown and overexpression experiments in ovarian cancer cells and Ba/F3 cells.
  • Analysis of cell viability, migration, proliferation, and apoptosis markers (cleaved PARP, Bcl-2).
  • Investigation of SPSB1's interaction with p21 and its effect on p21 degradation via ubiquitination and proteasomal pathways.

Main Results:

  • SPSB1 knockdown decreased ovarian cancer cell viability and migration.
  • Ectopic SPSB1 overexpression increased proliferation in Ba/F3 cells.
  • SPSB1 knockdown elevated p21 levels, induced apoptosis, and promoted p21 destabilization through ubiquitin-mediated proteasomal degradation.

Conclusions:

  • SPSB1 plays a significant role in promoting ovarian cancer cell proliferation and survival.
  • SPSB1 directly interacts with p21, accelerating its degradation and contributing to cancer progression.
  • These findings offer novel insights into SPSB1's function in ovarian cancer.
Keywords:
Cell survivalOvarian cancerSPSB1Ubiquitinationp21 destabilization

More Related Videos

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines11:50

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Published on: September 10, 2014

13.9K
Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens05:58

Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens

Published on: February 4, 2014

14.2K

Related Concept Videos

Cancer Survival Analysis01:21

Cancer Survival Analysis

Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...
756
Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.6K
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
3.8K
Survival Curves01:18

Survival Curves

Survival curves are graphical representations that depict the survival experience of a population over time, offering an intuitive way to track the proportion of individuals who remain event-free at each time point. These curves are widely used in fields such as medicine, public health, and reliability engineering to visualize and compare survival probabilities across different groups or conditions.
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
702
Survival Tree01:19

Survival Tree

Survival trees are a non-parametric method used in survival analysis to model the relationship between a set of covariates and the time until an event of interest occurs, often referred to as the "time-to-event" or "survival time." This method is particularly useful when dealing with censored data, where the event has not occurred for some individuals by the end of the study period, or when the exact time of the event is unknown.
 Building a Survival Tree
Constructing a...
422
Introduction To Survival Analysis01:18

Introduction To Survival Analysis

Survival analysis is a statistical method used to study time-to-event data, where the "event" might represent outcomes like death, disease relapse, system failure, or recovery. A unique feature of survival data is censoring, which occurs when the event of interest has not been observed for some individuals during the study period. This requires specialized techniques to handle incomplete data effectively.
The primary goal of survival analysis is to estimate survival time—the time...
779