Phenylobacterium parvum sp. nov., isolated from lake water

A Gram-stain-negative, rod-shaped and aerobic bacterium, designated HYN0004^T, was isolated from lake water. The strain grew at 15-35 °C and pH 7.0-9.0 on R2A. The isoprenoid quinone was Q10 and major polar lipids were phosphatidylglycerol and one unidentified glycolipid. The genome was 2.83 Mb with a DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analyses revealed that HYN0004^T represented a member of the genus Phenylobacterium and shared sequence similarities with Phenylobacterium conjunctum (97.8 %), Phenylobacterium koreense (97.5 %), Phenylobacterium aquaticum (97.2 %), and Phenylobacteriumheamatophilum (97.0 %). In addition to the low sequence similarities, the phylogenetic tree shapes indicated that HYN0004^Trepresents an independent species of this genus. The genomic and phenotypic properties, including small genome size, inability to carry out numerous enzymatic reactions and high ratio of C18 : 1ω6c and/or C18 : 1ω7c in fatty acids, verified the differentiation between HYN0004^T and related species. Thus, we propose a novel species of the genus Phenylobacterium, named as Phenylobacterium parvum sp. nov. The type strain is HYN0004^T (=KACC 19185^T=NBRC 112736^T).