Related Experiment Video

Updated: Jan 25, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
08:56

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

3.5K

Raman fingerprints as promising markers of cellular senescence and aging

Lisa Liendl1, Johannes Grillari1,2,3, Markus Schosserer4

  • 1Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, 1190, Vienna, Austria.

Geroscience
|February 5, 2019
PubMed

Insights

Understanding cellular aging is crucial. Raman microspectroscopy offers a promising method for detecting senescent cells, which accumulate with age and contribute to age-related diseases.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Spectroscopy

Background:

  • Cellular senescence, characterized by irreversible growth arrest and altered molecular profiles, increases with age.
  • Senescent cells contribute to age-related diseases, but their in vivo detection remains challenging.
  • Aging is a significant public health concern, necessitating deeper molecular understanding.

Purpose of the Study:

  • To review the applications of Raman microspectroscopy in studying aging.
  • To highlight the potential of Raman microspectroscopy for detecting senescent cells in vivo.
  • To provide an overview of Raman microspectroscopy's utility in aging research.

Main Methods:

  • Raman microspectroscopy enables the acquisition of biochemical fingerprints from cells and tissues.
  • This technique can differentiate between cellular states (e.g., senescent vs. non-senescent).
  • It has been used to investigate molecular changes during aging at cellular and tissue levels.

Main Results:

  • Raman microspectroscopy has demonstrated success in distinguishing senescent from non-senescent cells.
  • The technique provides insights into molecular alterations associated with cellular aging.
  • Successful applications in distinguishing healthy from cancerous tissues are also noted.

Conclusions:

  • Raman microspectroscopy is a valuable tool for investigating aging.
  • Its application in detecting senescent cells offers a novel approach for aging research.
  • Further exploration of Raman microspectroscopy in aging studies, particularly for in vivo senescent cell detection, is warranted.
Keywords:
BiomarkerCellular senescenceLabel-free imagingRaman microspectroscopySenolytic compoundsSkin aging

More Related Videos

Induction and Validation of Cellular Senescence in Primary Human Cells08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

18.0K
Techniques to Induce and Quantify Cellular Senescence06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

34.9K

Related Concept Videos

IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
1.9K
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.3K
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
643
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.5K
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.4K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.1K