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Updated: Jan 29, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
Published on: December 4, 2021
Isolation and characterization of a novel lutein-producing marine microalga using high throughput screening
1Food Science and Technology Department, Faculty of Agriculture, Alexandria University, 21545, El-Shatby, Alexandria, Egypt; Department of Materials Science & Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada.
Abstract:
A novel highly selective lutein-producing marine microalga was isolated and identified using high throughput screening (HTS). Phylogenetic analysis based on 18S rRNA gene sequence revealed it belongs to a microalgae class (Trebouxiophyceae), and is closely related to Auxenochlorella spp. The novel strain was designated as Auxenochlorella sp. strain LEU27. Based on HPLC-DAD and HPLC-MS analyses, strain LEU27 produced a total of 1203.9 ± 98 μg g-1 dry cells of carotenoids, including a remarkable amount (996.6 ± 98 μg g-1 dry cells) of pure lutein under heterotrophic growth conditions. This is the first report that describes a natural, highly selective lutein-producing marine microalga isolated by HTS approach.
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