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[A real-time fluorescence quantitative PCR method for source identification of Crocus sativus and Carthamus
Quan-Fang Zhang1, Yan-Yan Liu1, Qing-Qing Tan1
1Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Laboratory of Applied Life Science, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China.
Abstract:
The specific PCR primer was designed base on ITS2 sequence in GenBank, and we developed a SYBRGreen real-time fluorescence quantitative PCR system for identification of Crocus sativus and Carthamus tinctorius source. Compared with Chinese herbal medicine DNA barcode technique, this method showed characteristics of shorter time, higher specificity and sensitivity. Using this method to detect 15 samples, 4 were C. sativus, 8 were C. tinctorius, and the other 3 samples were none of them. The result was in accordance with Chinese herbal medicine DNA barcode. This study lays the foundation for identification of related Chinese medical materials.
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