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Updated: Jan 29, 2026

Production and Use of Customizable Agarose Molds for Scaffold-Free Mouse Ovarian Follicle Culture
Published on: October 24, 2025
Electrospun patterned porous scaffolds for the support of ovarian follicles growth: a feasibility study
Liliana Liverani1, Nathalie Raffel2, Amir Fattahi2
1Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany. liliana.liverani@fau.de.
Abstract:
Recently, the interest of the scientific community is focused on the application of tissue engineering approach for the fertility restoration. In this paper innovative patterned electrospun fibrous scaffolds were fabricated and used as 3D system for porcine follicles culture. The obtained scaffolds demonstrated to be a suitable support which did not alter or interfere with the typical spherical follicles morphology. The fibrillar structure of the scaffolds mimics the morphology of the healthy native tissue. The use of porcine follicles implied many advantages respect to the use of mouse model. Relevant results showed that more than the scaffold pattern and struts dimension, the selection of proper biomaterials improve the follicles adhesion and development.
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