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Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.

Lucas F Ribeiro¹, Vanesa Amarelle², Liliane F C Ribeiro³

¹Department of Biology, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, University of São Paulo, São Paulo, SP, Brazil.

Biomed Research International

|February 6, 2019

Summary

Protein engineering creates novel biosensors called protein switches that detect analytes without washing steps. Utilizing periplasmic binding proteins (PBPs) expands their sensing capabilities for diverse applications.

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Area of Science:

Biotechnology
Protein Engineering
Biosensor Development

Background:

Biosensing relies on analyte recognition and signal transduction.
Existing biosensors often require complex procedures like washing steps and expensive equipment.
Protein switches offer a promising alternative by integrating sensing and response functionalities.

Purpose of the Study:

To review protein engineering strategies for developing versatile protein switches.
To highlight the use of periplasmic binding proteins (PBPs) in expanding the recognition repertoire of molecular switches.
To discuss advancements in allosteric domain fusions for biosensor design.

Main Methods:

Engineering synthetic protein switches through gene fusion and domain insertion.
Utilizing periplasmic binding proteins (PBPs) as sensing components due to their ligand-binding diversity and conformational changes.
Applying protein engineering approaches to expand the functionality of protein switches.

Main Results:

Protein switches can be designed to respond to specific analytes, transitioning between 'on' and 'off' states.
The incorporation of PBPs significantly broadens the range of detectable molecules for biosensing.
Allosteric domain fusions are a key strategy in designing advanced protein-based biosensors.

Conclusions:

Protein switches represent a significant advancement in biosensor technology, overcoming limitations of traditional methods.
Periplasmic binding proteins are crucial for enhancing the specificity and versatility of engineered biosensors.
Continued protein engineering efforts are expanding the potential of molecular switches for various sensing applications.