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Related Concept Videos

Inductance: Single-Phase And Three-Phase Line01:28

Inductance: Single-Phase And Three-Phase Line

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Understanding the inductance of transmission lines is crucial for efficient design and operation in electrical power systems. This discussion delves into the inductance characteristics of single-phase two-wire and three-phase three-wire transmission lines with equal phase spacing.
Single-Phase Two-Wire Line:
A single-phase line consists of two solid cylindrical conductors, denoted as x and y. Each conductor carries phasor currents ix and iy, respectively. Given that the sum of these currents is...
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Capacitance: Single-Phase And Three-Phase Line01:25

Capacitance: Single-Phase And Three-Phase Line

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In electrical power systems, understanding the capacitance of transmission lines is fundamental for efficient operation.
Single-Phase Lines
Consider a single-phase, two-wire transmission line with equal phase spacing energized by a voltage source. One conductor carries a uniform positive charge, while the other carries an equal negative charge. The capacitance C of the line can be derived from the voltage V between the conductors. For a one-meter section of the line, the capacitance is given...
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Power Distribution in Three-phase and Single Phase Circuits01:17

Power Distribution in Three-phase and Single Phase Circuits

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Power distribution within electrical circuits is a foundational aspect of residential and industrial energy systems. While single-phase power is common in residential settings, three-phase power is the standard for industrial environments with heavy machinery. Each system is different and has advantages, and it's crucial to understand the underlying principles of power distribution and material efficiency.
Single-Phase Power Distribution:
Single-phase circuits are typical in household settings;...
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Atomic Structure01:33

Atomic Structure

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Overview
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Atomic Mass01:52

Atomic Mass

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Atoms — and the protons, neutrons, and electrons that compose them — are extremely small. For example, a carbon atom weighs less than 2 × 10−23 g. When describing the properties of tiny objects such as atoms, we use appropriately small units of measure, such as the atomic mass unit (amu). The amu was originally defined based on hydrogen, the lightest element, then later in terms of oxygen. Since 1961, it has been defined with regard to the most abundant isotope of carbon, atoms of which...
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Atomic Orbitals02:44

Atomic Orbitals

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An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Multi-ATOM: Ultrahigh-throughput single-cell quantitative phase imaging with subcellular resolution.

Kelvin C M Lee1, Andy K S Lau1, Anson H L Tang1

  • 1Department of Electrical and Electronic Engineering, Faculty of Engineering, The University of Hong Kong, Hong Kong.

Journal of Biophotonics
|February 6, 2019
PubMed
Summary
This summary is machine-generated.

Multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) enables high-throughput, label-free cell imaging without sacrificing resolution. This quantitative phase imaging breakthrough achieves >94% accuracy in classifying cell types for biological and diagnostic applications.

Keywords:
microfluidicsquantitative phase imagingsingle-cell imagingultrafast imaging

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Quantitative phase imaging (QPI) is valuable for label-free cell analysis.
  • Current QPI methods lack resolution at high throughput, limiting single-cell precision.
  • This hinders applications in large, heterogeneous cell populations.

Purpose of the Study:

  • Introduce a novel QPI modality, multi-ATOM, for ultrahigh-throughput, high-resolution imaging.
  • Overcome limitations of existing QPI techniques.
  • Enable accurate, large-scale cell classification.

Main Methods:

  • Developed multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM).
  • Employed ultrafast phase-gradient encoding for robust phase retrieval.
  • Achieved throughput exceeding 10,000 cells/sec without interferometry.

Main Results:

  • Multi-ATOM provides label-free, subcellular resolution imaging at ultrahigh throughput.
  • Demonstrated robust phase retrieval surpassing current QPI methods.
  • Achieved >94% accuracy in classifying cell types, including cancer subtypes.

Conclusions:

  • Multi-ATOM offers a powerful tool for large-scale biophysical single-cell analysis.
  • This technology can significantly advance biological research.
  • Potential applications include enhanced disease diagnostics.