Effects of varying radiation dosages on MMP1 expression, and MMP1 knockdown on the viability and migration of SW620...

Colorectal cancer (CRC), also known as bowel cancer, is one of the leading causes of cancer‑associated mortality worldwide at present. The aim of the present study was to detect the effects of matrix metalloproteinase 1 (MMP1) on the viability and migration of a CRC cell line in the presence or absence of variation X‑ray radiation doses. The CRC cell line, SW620, was cultured and treated with different X‑ray doses (0, 0.1, 0.5, 1, 3 and 6 Gy). MMP1 expression was downregulated via the application of a specific small interfering (si)‑RNA. The viability and migration of SW620 cells prior to and following transfection were detected with MTT and Transwell chamber assays, respectively. The application of siRNA transfection to silence MMP1 in SW620 cells resulted in reduced cell viability and migration (P<0.05). Compared with the control, the cell viability and migration of cells were significantly reduced when exposed to 0.5, 1, 3, and 6 Gy X‑ray radiation (P<0.05). In SW620 cells treated with different X‑ray doses, the mRNA expression levels of MMP1 were significantly reduced (P<0.05). Cells treated with 0.5 Gy X‑ray exposure exhibited the lowest mRNA expression levels of MMP1 when compared with other doses of X‑ray radiation. The expression of MMP1 was associated with the promotion of the viability and migration of SW620 cells. X‑ray radiation with 6 Gy dosages significantly reduced cell viability when compared with the control. Thus, MMP1‑targeted therapy combined with radiotherapy could be used for treating CRC.