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Updated: Jan 29, 2026

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
MicroRNA-625-3p promotes cell migration of oral squamous cell carcinoma by regulating SCAI expression
1Department of Stomatology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou City, Changzhou, China. gumin106@163.com.
Objective:
The aim of this study was to investigate the role of microRNA-625-3p in the occurrence and progression of oral squamous cell carcinoma (OSCC) and its underlying mechanism.
Patients And Methods:
Expression levels of microRNA-625-3p, SCAI and E-cadherin in OSCC tissues and paracancerous tissues were detected by quantitative real time-polymerase chain reaction (qRT-PCR). MicroRNA-625-3p expression in OSCC tissues with different tumor stages and lymph node metastasis stages was analyzed. Survival analyses were conducted to access the diagnostic values of microRNA-625-3p and SCAI in OSCC. The effect of microRNA-625-3p on regulating cell migration of OSCC was detected by transwell assay. Luciferase reporter gene assay was conducted to verify the binding condition between microRNA-625-3p and SCAI. Rescue experiments were performed by co-transfection of microRNA-625-3p inhibitor and si-SCAI, followed by cell proliferation detection.
Results:
MicroRNA-625-3p was highly expressed in OSCC tissues than that of paracancerous tissues. OSCC patients with T3+T4 presented higher expression of microRNA-625-3p than those with T1+T2. Similarly, OSCC patients with N1+N2 presented higher expression of microRNA-625-3p than those with N0. Luciferase reporter gene assay identified that SCAI is the target gene of microRNA-625-3p. Furthermore, we found that SCAI and E-cadherin are lowly expressed in OSCC tissues than that of paracancerous tissues. ROC curve showed that microRNA-625-3p and SCAI exert certain values in diagnosing OSCC. MicroRNA-625-3p promoted migration of OSCC cells, which was reversed by SCAI knockdown.
Conclusions:
MicroRNA-625-3p is highly expressed in OSCC, which promotes cell migration of OSCC by regulating SCAI expression.
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