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Long non-coding RNA Tubulin Alpha 4B (TUBA4B) inhibited breast cancer proliferation and invasion by directly

A-X Liu1, F Yang, L Huang

  • 1Department of Oncology, Zhujiang Hospital, Southern Medical University, GuangZhou City, P.R. China. Zhangjiren@126.com.

European Review for Medical and Pharmacological Sciences
|February 6, 2019
PubMed
Abstract

Insights

Long non-coding RNA Tubulin Alpha 4B (TUBA4B) is downregulated in breast cancer. Overexpression of TUBA4B inhibits breast cancer cell proliferation and invasion by targeting miR-19, suggesting a tumor-suppressive role.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are key regulators in tumor development.
  • Tubulin Alpha 4B (TUBA4B) is implicated in cancer, but its role in breast cancer is undefined.

Purpose of the Study:

  • To investigate the expression and function of TUBA4B in breast cancer.
  • To elucidate the molecular mechanism of TUBA4B in breast cancer progression.

Main Methods:

  • Quantified TUBA4B expression in breast cancer tissues and cell lines.
  • Assessed the impact of TUBA4B on cell proliferation and invasion using CCK8 and Transwell assays.
  • Identified direct targets of TUBA4B using luciferase reporter assays.

Main Results:

  • TUBA4B expression was significantly downregulated in breast cancer samples and cell lines.
  • Overexpression of TUBA4B suppressed breast cancer cell proliferation and invasion.
  • TUBA4B was found to directly target miR-19, which mediated its tumor-suppressive effects.

Conclusions:

  • TUBA4B acts as a tumor suppressor in breast cancer.
  • The downregulation of TUBA4B contributes to breast cancer progression.
  • Targeting the TUBA4B/miR-19 axis may offer a therapeutic strategy for breast cancer.

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