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Effects of miR-153 on angiogenesis in MCAO rats through Shh signaling pathway

B-X Wang1, J-J Xu, J Hu

  • 1Department of Neurology, The First Hospital of Jiaxing, The First Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. cjmcofnggpmraa@sina.com.

European Review for Medical and Pharmacological Sciences
|February 6, 2019
PubMed
Abstract

Insights

MicroRNA-153 (miR-153) promotes angiogenesis and improves outcomes in middle cerebral artery occlusion (MCAO) rats by activating the Sonic Hedgehog (Shh) pathway. This suggests miR-153 is a potential therapeutic target for ischemic stroke.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Cerebrovascular disease is a leading cause of death in China.
  • Angiogenesis post-cerebral infarction impacts patient prognosis.
  • MicroRNAs (miRNAs) regulate neovascularization; miR-153 shows protective effects in ischemic injury, but its role in the Sonic Hedgehog (Shh) pathway is unknown.

Purpose of the Study:

  • To investigate the role of miR-153 in angiogenesis in middle cerebral artery occlusion (MCAO) rat models.
  • To explore the involvement of the Shh signaling pathway in miR-153-mediated angiogenesis.

Main Methods:

  • Established MCAO rat model, treated with Agomir-153 and 5-EI.
  • Quantified miR-153 expression via RT-PCR.
  • Assessed neurological function, infarct area (TTC), and capillary density.
  • Analyzed Shh pathway and angiogenesis factors using Western blot.

Main Results:

  • Agomir-153 increased miR-153 expression, reduced infarct size, and enhanced cerebral capillary formation in MCAO rats.
  • 5-EI partially reversed Agomir-153's pro-angiogenic and protective effects.
  • Upregulated miR-153 inhibited patched (PTC) expression and activated the Shh signaling pathway.

Conclusions:

  • Upregulated miR-153 activates the Shh pathway, promoting angiogenesis and improving prognosis in MCAO rats via lipid-coated Patch (PTC).
  • MiR-153 represents a novel therapeutic target for enhancing angiogenesis after MCAO.

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