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Updated: Jan 29, 2026

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
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Macrophage migration inhibitory factor antagonist (p425) ameliorates kidney histopathological and functional changes

Jamal Khalilpour1, Shiva Roshan-Milani1,2, Farzaneh Hosseini Gharalari3,4

  • 1Urmia University of Medical Sciences, Department of Physiology, Faculty of Medicine, Urmia, Iran.

Jornal Brasileiro De Nefrologia
|February 6, 2019
PubMed
Abstract

Insights

Inhibition of macrophage migration inhibitory factor (MIF) protected against diabetic nephropathy (DN) in rats. MIF antagonist treatment reduced kidney damage and improved renal function in a diabetic model.

Area of Science:

  • Nephrology
  • Immunology
  • Endocrinology

Background:

  • Macrophage migration inhibitory factor (MIF) is implicated in diabetic nephropathy (DN) pathogenesis.
  • Investigating MIF's role in DN is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To evaluate the therapeutic potential of MIF inhibition in a rat model of DN.
  • To assess the renal effects of a specific MIF antagonist (p425) in diabetic conditions.

Main Methods:

  • Streptozotocin-induced diabetic Wistar rats were treated with MIF antagonist (p425).
  • Urine albumin excretion, biochemical markers (BUN, Cr), and histological changes were analyzed.
  • Treatment commenced upon observing increased urine albumin excretion in diabetic rats.

Main Results:

  • MIF antagonist (p425) significantly reduced urine protein and GAG excretion.
  • Treatment lowered urine protein/creatinine ratio and serum BUN and creatinine levels.
  • Histopathological analysis revealed significant alleviation of kidney damage in treated rats.

Conclusions:

  • MIF antagonist (p425) demonstrates protective effects against functional and histopathological kidney injury in DN.
  • Targeting MIF presents a potential therapeutic strategy for managing diabetic nephropathy.

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