Novel cone-and-plate flow chamber with controlled distribution of wall fluid shear stress

Fluid flow in blood vessels or interstitial fluid flow within tissue cavities plays important roles in tissue regeneration. One of the fundamental issues for in vitro study of the effects of fluid shear stress (FSS) on cells is the development of a flow chamber that can provide a controlled FSS field. In this study, we developed a novel cone-and-plate flow chamber based on viscometry technology, in which the cone's shape was optimized to produce a uniform wall FSS field on the surface of a standard six-well cell culture plate. By using a FSS finite element method, the effects of different geometric parameters of cone and plate, viscosity coefficient of fluid, and angular velocity on wall FSS at the bottom surface of the culture plate were investigated. Results of the simulation demonstrated that the cone with polyline or truncated generatrix (TG) could produce wall FSS as high as 1 or 2 Pa with uniform distribution, in which the area of the identical region for the cone with TG accounts for more than 69% of the total area. In addition, with the cone in close proximity to the plate surface, a gap distance of 0.1 mm can produce a uniform FSS field with a magnitude as high as 2 Pa over the majority of the plate. Furthermore, particle image velocimetry was utilized to measure the distribution of wall FSS, through which the numerical simulation results were experimentally demonstrated. This study presents a powerful new device for in vitro fluid flow loading at the cellular level.