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Updated: Jan 29, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
Quantitative analysis of Alaska pollock in seafood products by droplet digital PCR
Eun Soo Noh1, Yeon Jung Park1, Eun Mi Kim1
1Biotechnology Research Division, National Institute of Fisheries Science, 216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, Republic of Korea.
Abstract:
A highly accurate quantitative method, based on the new technique, droplet digital PCR (ddPCR), was applied to determine the content of Alaska pollock (Gadus chalcogrammus) in seafood products. Using this method, we found a linear relationship among raw sample weight, DNA concentration and DNA copy number. We also established a formula to calculate the raw sample weight, based on the number of DNA copies. To confirm the accuracy and applicability of this method, mixed samples of known composition were analyzed. Results from this study indicated that the ddPCR method described is suitable for quantifying Alaska pollock in seafood products and has the potential applied to a variety of tasks in food quality certification.
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