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Pectobacterium aquaticum sp. nov., isolated from waterways
Jacques Pédron1, Claire Bertrand1, Géraldine Taghouti2
11Sorbonne Université, INRA, Institute of Ecology and Environmental sciences-Paris, 4 place Jussieu, F-75 252 Paris, France.
International Journal of Systematic and Evolutionary Microbiology
|February 7, 2019
Summary
Six novel bacterial strains from French waterways represent a new species, Pectobacterium aquaticum. This discovery expands the Pectobacterium genus and aids in understanding aquatic microbial diversity.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Pectobacterium comprises plant-pathogenic bacteria, with ongoing research to delineate species and understand their ecological roles.
- Accurate taxonomic classification is crucial for disease management and ecological studies.
Purpose of the Study:
- To determine the taxonomic status of six Pectobacterium strains isolated from French waterways.
- To investigate if these strains represent a novel species within the Pectobacterium genus.
Main Methods:
- Phylogenetic analysis using the gapA housekeeping gene.
- Phenotypic characterization using GENIII Biolog plates.
- Whole-genome sequencing (Illumina) followed by phylogenetic data analysis, average nucleotide identity (ANI), and in silico DNA-DNA hybridization (dDDH).
Main Results:
- Phylogenetic analysis placed the six strains within the Pectobacterium genus, distinct from known species.
- Phenotypic analysis showed weak metabolic activity at pH 5 for the novel strains.
- Phylogenetic data, ANI, and dDDH values supported the designation of these strains as a new species.
Conclusions:
- The six strains represent a novel species, Pectobacterium aquaticum sp. nov.
- The proposed type strain is A212-S19-A16T.
- This finding contributes to the taxonomic diversity of the Pectobacterium genus in aquatic environments.
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