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A NOVEL BIOLOGICAL DOSIMETRY ASSAY AS A POTENTIAL TOOL FOR TRIAGE DOSE ASSESSMENT IN CASE OF LARGE-SCALE RADIOLOGICAL
A Testa1, V Palma1, C Patrono1
1ENEA Casaccia Research Center, Department for Sustainability, Division Health Protection Technologies, Via Anguillarese, 301 Rome, Italy.
Radiation Protection Dosimetry
|February 7, 2019
Summary
A new cytogenetic assay combines dicentric and micronucleus methods for efficient biological dosimetry in radiological emergencies. This time-saving, potentially automatable tool aids in rapid casualty triage.
Area of Science:
- Cytogenetics
- Radiation Biology
- Biomedical Engineering
Background:
- Efficient triage of casualties in mass radiological emergencies requires advanced biological and clinical endpoints.
- Current biological dosimetry methods, while established, can be time-consuming and resource-intensive for large-scale events.
Purpose of the Study:
- To develop a novel, integrated cytogenetic assay for rapid biological dosimetry in mass radiological emergencies.
- To create a time-saving, cost-effective, and potentially automatable method for casualty triage.
- To enable simultaneous scoring of chromosome aberrations and micronuclei, distinguishing cell division stages without specialized staining.
Main Methods:
- Development of a combined dicentric and micronucleus assay protocol.
- Simultaneous scoring of chromosome aberrations and micronuclei on single slides.
- Discrimination of metaphase stages (first and second cell division) without Fluorescence plus Giemsa staining.
Main Results:
- A novel 'three-in-one' cytogenetic assay was developed.
- The assay allows simultaneous scoring of dicentric chromosomes, micronuclei, and cell division stages.
- The method eliminates the need for Fluorescence plus Giemsa staining for stage discrimination.
Conclusions:
- The developed assay offers a promising, integrated approach for biological dosimetry in mass radiological events.
- Further validation through inter-laboratory comparisons is necessary to confirm reproducibility.
- Investigating the compatibility with existing automation software is recommended for practical implementation.
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