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Micropropagation of Spathoglottis plicata
W-L Teng1, L Nicholson1, M-C Teng2
1Hong Kong Institute of Biotechnology, 2 Biotechnology Avenue, 12 Miles, Tai Po Road, Shatin, N. T., Hong Kong E-mail: wl-teng@hkib.org.hk, , , , , , HK.
Plant Cell Reports
|February 8, 2019
Summary
This study details a rapid micropropagation technique for Spathoglottis plicata using specific plant growth regulator combinations. Nodal explants proved highly effective for regenerating protocorm-like bodies and plantlets.
Area of Science:
- Plant Biotechnology
- Horticultural Science
- Plant Tissue Culture
Background:
- Micropropagation is crucial for rapid multiplication of orchids.
- Spathoglottis plicata is an orchid species with horticultural value.
- Efficient propagation methods are needed for Spathoglottis plicata.
Purpose of the Study:
- To establish a rapid and reliable micropropagation protocol for Spathoglottis plicata.
- To determine optimal plant growth regulator (PGR) concentrations and ratios for Spathoglottis plicata regeneration.
- To evaluate the efficacy of different explant types for micropropagation.
Main Methods:
- Nodal and leaf explants from 8-month-old Spathoglottis plicata seedlings were cultured.
- Murashige and Skoog medium with charcoal and 16 NAA/BA combinations (0.54-10.74 µM) were used.
- Regeneration of protocorm-like bodies (PLBs) and plantlets was assessed.
Main Results:
- Nodal explants showed 98.5% regeneration of PLBs and plantlets.
- Optimal PGR for PLB regeneration: 5.37 µM NAA and 0.44 µM BA (NAA:BA ratio 12.2).
- Optimal PGR for plantlet development: 2.69-10.74 µM NAA and 8.88 µM BA (NAA:BA ratio 0.3-1.2).
Conclusions:
- Nodal explants are superior for Spathoglottis plicata micropropagation.
- Specific NAA and BA concentrations and ratios significantly influence regeneration and development.
- Subculturing small PLB/plantlet pieces enables continuous regeneration.
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