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Updated: Jan 29, 2026

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
Published on: December 19, 2014
Transformation of lisianthus (Eustoma grandiflorum)
S E Ledger1, S C Deroles1, D G Manson1
1Levin Research Centre, Crop and Food Research, Private Bag 4005, Levin, New Zealand Fax no.: 64-6-368-3578; E-mail: Deroless@crop.cri.nz, , , , , , NZ.
Abstract:
Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase II. pKIWI110 also contains the reporter gene for β-D-glucuronidase, and pLN26, the chalcone synthase antisense gene. Southern DNA analysis revealed that all the kanamycin-resistant transformants tested contained copies of the transgenes integrated in their genome. The two plants transformed with pKIWI110 show β-D-glucuronidase expression in their mature leaves and selected transformants passed on the kanamycin-resistant phenotype to the F1 generation.
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